Protocols

The protocols listed below were optimized and tweaked to our liking. We suggest others to try according to our protocol first and then tweak it to their lab conditions, if necessary.

Annealing Oligos

Bleaching Embryos

Designing posters

Ethanol precipitation

Extraction of genomic DNA from zebrafish embryos for Southern Blotting

Extraction of genomic DNA from zebrafish embryos for PCR

Fishroom To-Do List

Gateway Cloning

Generation of riboprobes

Generation of stable cell lines

How to submit DNAs for sequencing

Immunofluorescence Cell Staining

Inoue method for preparation of competent E. coli

In situ hybridization

In vitro transcription

Mating Fish

miRNA in situ hybridization

PCR Cloning

Preparation of electrocompetent cells

Preparation of embryos for WB

Simple Cut & Paste

Site-directed mutagenesis

Tetratest ammonia kit

Transfection of miRNA into mammalian monolayered cells

Tricaine & PTU

Western Blotting of Zebrafish Embryos