Lab Publications
Featured
For a full list of publications see below.
We summarize representative transgenic fuorescent zebrafsh lines that have revolutionized biomedical research on signal transduction, the craniofacial skeletal system, the hematopoietic system, the nervous system, the urogenital system, the digestive system and intracellular organelles
Choe CP, Choi SY, Kee Y, Kim MJ, Kim SH, Lee Y, Park HC, Ro H.
Here we show that zebrafish embryos deficient in Wnt signaling have defective motile cilia, yet harbor intact basal bodies. With respect to maintenance of ependymal motile cilia, plcδ3a is a target gene of Wnt signaling. Lack of Connexin43 (Cx43), especially its channel function, decreases motile cilia and intercellular Ca2+ wave (ICW) propagation. Genetic ablation of cx43 in zebrafish and mice diminished motile cilia. Finally, Cx43 is also expressed in ECs of the human SC. Taken together, our findings indicate that gap junction mediated ICWs play an important role in the maintenance of ependymal motile cilia, and suggest that the enhancement of functional gap junctions by pharmacological or genetic manipulations may be adopted to ameliorate motile ciliopathy.
Zhang J, Chandrasekaran G, Li W, Kim DY, Jeong IY, Lee SH, Liang T, Bae JY, Choi I, Kang H, Maeng JS, Kim MK, Lee T, Park SW, Kim MJ, Kim HS, Ro H, Bae YC, Park HC, Choi EY*, Choi SY*.
A new series of 1,3-diketone, heterocyclic and α,β-unsaturated derivatives were synthesized and evaluated for their AhR antagonist activity using zebrafish and mammalian cells. Compounds 1b, 2c, 3b and 5b showed significant AhR antagonist activity in a transgenic zebrafish model. Among them, compound 3b, and 5b were found to have excellent AhR antagonist activity with IC50 of 3.36 nM and 8.3 nM in a luciferase reporter gene assay. In stem cell proliferation assay, compound 5b elicited marked HSC expansion.
Jeong J, Kim KH, Kim DY, Chandrasekaran G, Kim M, Pagire SH, Dighe M, Choi EY, Bak SM, Kim EY, Shin MG*, Choi SY*, Ahn JH*.
We found a GFAP mutation (c.382G > A, p.Asp128Asn) in a 68-year-old man with slowly progressive gait disturbance with tendency to fall. The patient was tentatively diagnosed with AxD based on clinical and radiological findings. The p.Asp128Asn GFAP mutation is likely to be a disease-causing mutation. Although it needs to be tested more extensively in larger case series, the zebrafish assay system presented here would help clinicians determine whether GFAP mutations identified in putative AxD patients are disease-causing.
Lee SH, Nam TS, Kim KH, Kim JH, Yoon W, Heo SH, Kim MJ, Shin BA, Perng MD, Choy HE, Jo J*, Kim MK*, Choi SY*.
We sought to determine tissues that are vulnerable to TCDD toxicity using a transgenic zebrafish (Danio rerio) model. Expression pattern of EGFP in the reporter zebrafish mirrored that of endogenous cyp1a mRNA. Exposure of the embryos to TCDD revealed previously reported (the cardiovascular system, liver, pancreas, kidney, swim bladder and skin) and unreported target tissues (retinal bipolar cells, otic vesicle, lateral line, cloaca and pectoral fin bud) for TCDD. Transgenic cyp1a reporter zebrafish we have developed can further help to understand the ecotoxicological relevance and human health risks by TCDD. In addition, they could be used to identify agonists of AhR and antidotes to TCDD toxicity.
Kim KH, Park HJ, Kim JH, Kim SH, Williams DR, Kim MK, Jung YD, Teraoka H, Park HC, Choy HE, Shin BA, Choi SY.
. Here, we generated four expression plasmids each harboring different 2A peptides derived from the foot-and-mouth disease virus, equine rhinitis A virus, Thosea asigna virus and porcine teschovirus-1, respectively, and evaluated their cleavage efficiency in three commonly used human cell lines, zebrafish embryos and adult mice. Western blotting and confocal microscopic analyses revealed that among the four 2As, the one derived from porcine teschovirus-1 (P2A) has the highest cleavage efficiency in all the contexts examined. We anticipate that the 2A-harboring cloning vectors we generated and the highest efficiency of the P2A peptide we demonstrated would help biomedical researchers easily adopt the 2A technology when bicistronic or multicistronic expression is required.
Kim JH, Lee SR, Li LH, Park HJ, Jeong-Hoh Park, Lee KY, Kim MK, Shin BA and Choi SY.
Full List of publications
A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish.
Jang JY, Liang T, Kim MK, Kang KW, Lee B, Choi SY.
Zebrafish. 2022 Jul 26. Epub ahead of print.
A novel in-frame GFAP p.E138_L148del mutation in Type II Alexander disease with atypical phenotypes.
Kang YR, Lee SH, Lin NH, Lee SJ, Yang AW, Chandrasekaran G, Kang KW, Jin MS, Kim MK, Perng MD, Choi SY.
Eur. J. Hum. Genet. 2022 Mar 4:1-8.
Transgenic fluorescent zebrafish lines that have revolutionized biomedical research.
Choe CP, Choi SY, Kee Y, Kim MJ, Kim SH, Lee Y, Park HC, Ro H.
Lab Anim. Res. 2021 Dec;37(1):1-29.
Loss of splicing factor IK impairs normal skeletal muscle development.
Ka HI, Seo H, Choi Y, Kim J, Cho M, Choi SY, Park S, Han S, An J, Chung HS, Yang Y.
BMC Biol. 2021 Dec;19(1):1-7.
Fulminant Course of Neuromyelitis Optica in a Patient With Anti-MDA5 Antibody-Positive Dermatomyositis: A Case Report.
Kang YR, Kim KH, Nam TS, Lee KH, Kang KW, Lee SJ, Choi SY, Chandrasekaran G, Kim MK.
Front. Med. 2020 Nov 11;7:576436.
ACOX3 Dysfunction as a Potential Cause of Recurrent Spontaneous Vasospasm of Internal Carotid Artery.
Kim JT, Won SY, Kang K, Kim SH, Park MS, Choi KH, Nam TS, Denis SW, Ferdinandusse S, Lee JE*, Choi SY*, Kim MK*.
Transl. Stroke Res. 2020 Oct;11(5):1041-1051.* co-corresponding authors
Wnt-PLC-IP3-Connexin-Ca2+ axis maintains ependymal motile cilia in zebrafish spinal cord.
Zhang J, Chandrasekaran G, Li W, Kim DY, Jeong IY, Lee SH, Liang T, Bae JY, Choi I, Kang H, Maeng JS, Kim MK, Lee T, Park SW, Kim MJ, Kim HS, Ro H, Bae YC, Park HC, Choi EY*, Choi SY*.
Nat. Commun. 2020 Apr 20;11(1):1860.* co-corresponding authors
A zebrafish model of nondystrophic myotonia with sodium channelopathy.
Nam TS, Zhang J, Chandrasekaran G, Jeong IY, Li W, Lee SH, Kang KW, Maeng JS, Kang H, Shin HY, Park HC, Kim S*, Choi SY*, Kim MK*.
Neurosci. Lett. 2020 Jan 1;714:134579.* co-corresponding authors
Genetic characteristics of non-familial epilepsy.
Kang KW, Kim W, Cho YW, Lee SK, Jung KY, Shin W, Kim DW, Kim WJ, Lee HW, Kim W, Kim K, Lee SH, Choi SY*, Kim MK*.
PeerJ. 2019 Dec 19;7:e8278.* co-corresponding authors
Identification of new aryl hydrocarbon receptor (AhR) antagonists using a zebrafish model.
Jeong J, Kim KH, Kim DY, Chandrasekaran G, Kim M, Pagire SH, Dighe M, Choi EY, Bak SM, Kim EY, Shin MG*, Choi SY*, Ahn JH*.
Bioorg. Med. Chem. 2019 Oct 1;27(19):115014.* co-corresponding authors
Hyaluronan-Stabilized Redox-Sensitive Nanoassembly for Chemo-Gene Therapy and Dual T1/T2 MR Imaging in Drug-Resistant Breast Cancer Cells.
Rajendrakumar SK, Venu A, Revuri V, George Thomas R, Thirunavukkarasu GK, Zhang J, Vijayan V, Choi SY, Lee JY, Lee YK, Jeong YY, Park IK.
Mol. Pharm. 2019 May 6;16(5):2226-2234.
Teaching NeuroImages: Alexander disease with features of both frontal and bulbospinal involvement.
Nam TS, Kang KW, Choi SY, Kim MK.
Neurology. 2018 Jul 24;91(4):e396-e397.
A 3D-Printed Sensor for Monitoring Biosignals in Small Animals.
Cho SJ, Byun D, Nam TS, Choi SY, Lee BG, Kim MK, Kim S.
J. Healthc. Eng. 2017 Oct 25;2017.
A Novel GFAP Mutation in Late-Onset Alexander Disease Showing Diffusion Restriction.
Nam TS, Oh J, Levy M, Kang KW, Choi SY, Kim MK.
J. Clin. Neurol. 2017 Oct;13(4):426-428.
Aggregation-prone GFAP mutation in Alexander disease validated using a zebrafish model.
Lee SH, Nam TS, Kim KH, Kim JH, Yoon W, Heo SH, Kim MJ, Shin BA, Perng MD, Choy HE, Jo J*, Kim MK*, Choi SY*.
BMC Neurol. 2017 Sep 7;17(1):175.* co-corresponding authors
Functional validation of novel MKS3/TMEM67 mutations in COACH syndrome.
Lee SH, Nam TS, Li W, Kim JH, Yoon W, Choi YD, Kim KH, Cai H, Kim MJ, Kim C, Choy HE, Kim N, Chay KO*, Kim MK*, Choi SY*.
Sci. Rep. 2017 Aug 31;7(1):10222.* co-corresponding authors
Zebrafish as an animal model in epilepsy studies with multichannel EEG recordings.
Cho SJ, Byun D, Nam TS, Choi SY, Lee BG, Kim MK, Kim S.
Sci. Rep. 2017;7(1):3099
Novel NTRK1 mutations associated with congenital insensitivity to pain with anhidrosis verified by functional studies.
Nam TS, Li W, Yoon S, Eom GH, Kim MK*, Jung ST*, Choi SY*.
J. Peripher. Nerv. Syst. 2017 Jun;22(2):92-99.* co-corresponding authors
Development of a vestibular schwannoma xenograft zebrafish model for in vivo antitumor drug screening.
Lee HJ, Yang YJ, Jeong S, Lee JD, Choi SY, Jung DW, Moon IS.
The Laryngoscope. 2016 Dec;126(12):E409-E415.
Familial pachygyria in both genders related to a DCX mutation.
Kim YO, Nam TS, Park C, Kim SK, Yoon W, Choi SY, Kim MK, Woo YJ.
Brain Dev. 2016 Jun 1;38(6):585-9.
miRNA-105 and -128 function as rheostats modulating MMP-2 activities by downregulation of TIMP-2 and upregulation of MT1-MMP.
Kim JH, Li LH, Cai H, Nguyen VH, Min JJ, Shin BA*, Choi SY*, Koh YS*.
Genes Genom. 2016 Feb;38(2):217-23.* co-corresponding authors
cyp1a reporter zebrafish reveals target tissues for dioxin.
Kim KH, Park HJ, Kim JH, Kim SH, Williams DR, Kim MK, Jung YD, Teraoka H, Park HC, Choy HE, Shin BA, Choi SY.
Aquat. Toxicol. 2013 Jun 15;134:57-65.
An algorithm for candidate sequencing in non-dystrophic skeletal muscle channelopathies.
Nam TS, Lossin C, Kim DU, Kim MK, Kim YO, Choi KH, Choi SY, Park SC, Na IS.
J. Neurol. 2013 Jul;260(7):1770-7.
Clinical characteristics and analysis of CLCN1 in patients with "EMG disease."
Nam TS, Jung HJ, Choi SY, Kim YO, Kim MK, Cho KH.
J. Clin. Neurol. 2012 Sep 1;8(3):212-7.
Altered fast and slow inactivation of the N440K Nav1.4 mutant in a periodic paralysis syndrome.
Lossin C, Nam TS, Shahangian S, Rogawski MA, Choi SY*, Kim MK*, Sunwoo IN.
Neurology. 2012 Sep 4;79(10):1033-40.* co-corresponding authors
A novel zebrafish human tumor xenograft model validated for anti-cancer drug screening.
Jung DW, Oh ES, Park SH, Chang YT, Kim CH, Choi SY, Williams DR.
Mol. BioSyst. 2012 Jul 6;8(7):1930-9.
Histone methyltransferase SETD3 regulates muscle differentiation.
Eom GH, Kim KB, Kim JH, Kim JY, Kim JR, Kee HJ, Kim DW, Choe N, Park HJ, Son HJ, Choi SY, Kook H, Seo SB.
J. Biol. Chem. 2011 Oct 7;286(40):34733-42.
High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell liness, zebrafish and mice.
Kim JH, Lee SR, Li LH, Park HJ, Jeong-Hoh Park, Lee KY, Kim MK, Shin BA and Choi SY.
PLoS One. 2011 Apr 29;6(4):e18556.
piRNA-Associated Germline Nuage Formation and Spermatogenesis Require MitoPLD Profusogenic Mitochondrial-Surface Lipid Signaling.
Huang H, Gao Q, Peng X, Choi SY, Sarma K, Ren H, Morris AJ, Frohman MA.
Dev. Cell. 2011 Mar 15;20(3):376-87.
A novel cell-free mitochondrial fusion assay amenable for high-throughput screenings of fusion modulators.
Schauss AC, Huang H, Choi SY, Xu L, Soubeyrand S, Bilodeau P, Zunino R, Rippstein P, Frohman MA, McBride HM.
BMC Biol. 2010 Dec;8(1):1-2.
A quantitative assay for mitochondrial fusion using Renilla luciferase complementation.
Huang H, Choi SY, Frohman MA.
Mitochondrion. 2010 Aug 1;10(5):559-66.
Cardiolipin synthesis is required to support human cholesterol biosynthesis from palmitate upon serum removal in Hela cells.
Hauff KD, Choi SY, Frohman MA, Hatch GM.
Can. J. Physiol. Pharmacol. 2009 Oct;87(10):813-20.
Ascorbic Acid Suppresses the 2,3,7,8-Tetrachloridibenxo-p-dioxin (TCDD)-induced CYP1A1 Expression in Human HepG2 Cells.
Chang HJ, Park JS, Lee EK, Kim MH, Baek MK, Kim HR, Jeong HG, Choi SY, Jung YD.
Toxicol In Vitro. 2009 Jun 1;23(4):622-6.
Methanol extract of Elaeagnus glabra, a Korean medicinal plant, inhibits HT1080 tumor cell invasion.
Li LH, Baek IK, Kim JH, Kang KH, Koh YS, Jung YD, Cho CK, Choi SY* and Shin BA*.
Oncol. Rep. 2009 Feb 1;21(2):559-63.* co-corresponding authors
Real-time imaging of mitochondria in transgenic zebrafish expressing mitochondrially targeted GFP.
Kim MJ, Kang KH, Kim CH, Choi SY.
Biotechniques. 2008 Sep;45(3):331-4.
Cardiolipin deficiency releases cytochrome c from the inner mitochondrial membrane and accelerates stimuli-elicited apoptosis.
Choi SY, Gonzalvez F, Jenkins GM, Slomianny C, Chretien D, Arnoult D, Petit PX, Frohman MA.
Cell Death Differ. 2007 Mar;14(3):597-606.
A role for Phospholipase D in Drosophila embryonic cellularization.
LaLonde M, Janssens H, Yun S, Crosby J, Redina O, Olive V, Altshuller YM, Choi SY, Du G, Gergen JP, Frohman MA.
BMC Dev. Biol. 2006 Dec;6(1):1-3.
A common lipid links Mfn-mediated mitochondrial fusion and SNARE-regulated exocytosis.
Choi SY, Huang P, Jenkins GM, Chan DC, Schiller J, Frohman MA.
Nat. Cell Biol. 2006 Nov;8(11):1255-62.
Regulation of phototransduction responsiveness and retinal degeneration by a phospholipase D-generated signaling lipid.
LaLonde MM, Janssens H, Rosenbaum E, Choi SY, Gergen JP, Colley NJ, Stark WS, Frohman MA.
J. Cell Biol. 2005 May 9;169(3):471-9.